In vitro Synergistic effect of Cassia fistula leaves extracts with Fluconazole against clinical isolates of Candida albicans

نویسنده

  • Yogesh Bansal
چکیده

The aim of the study was to evaluate the synergistic antifungal action of crude DMSO (Di methyl sulphoxide) & Petroleum ether leaves extract of Cassia fistula in conjunction with fluconazole against candidial species isolated from Candidasis patients. Correlation was observed between the leaves & fluconazole, therefore it was also found that the antifungal action of Cassia fistula leaves extract was enhanced (up to 3 times) in combination with fluconazole on fluconazole resistance Candida albicans strains isolated from patients with Candidiasis. The plant appears to play an important role in overcoming the azole resistant of Candidial strains. IC50 was determined by agar well diffusion and broth dilution method in the presence of fluconazole and leaves extracts of Cassia fistula in different solvents. Synergistic behavior of leaves extracts in Pet. Ether and DMSO extract of Cassia fistula with fluconazole was checked by broth dilution method.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Inhibitory Effect of Fluconazole Combined with Amphotericin B on Fluconazole-Resistant Candida albicans Biofilm Formation

 Background & Objective:  The incidence of biofilm-related infections caused by Candida albicans has increased dramatically. C. albicans biofilm-related infections are more resistant to antifungal medications. This work was an attempt to examine inhibitory effects of fluconazole in combination with amphotericin B on fluconazole-resistant C. albicans biofilm.  Materials & Methods:  Fluconazole-...

متن کامل

In vitro Antifungal Effect of Aqueous Garlic (Allium Sativum) Extract and its Combination with Fluconazole Against Five Common Clinical Candida Isolated from Candidiasis Lesions

Introduction: Increasing rate of candidiasis prevalence and consequently use of antifungal drugs as prophylactic and curative agents has led to the widespread emergence of resistant strains. Therefore this study was designed to evaluate the in vitro antifungal activity of an aqueous extract of garlic and the synergic effect of garlic extract with fluconazole against common clinical isolates of ...

متن کامل

Anti-Candida albicans biofilm activity by Cassia spectabilis standardized methanol extract: an ultrastructural study.

BACKGROUND AND OBJECTIVE Candida (C.) albicans infection in its biofilm mode of growth has taken centre point with the increasing recognition of its role in human infections due to the development of resistance to the commonly used antibiotic or phenotypic adaptation within the biofilm. Hence, in this study the inhibitory effect of methanol extract of Cassia (C.) spectabilis leaves was evaluate...

متن کامل

In vitro antifungal activities of Euphorbia macroclada and fluconazole against pathogenic Candida species

Background and Purpose: Candida species constitute an important group of opportunistic fungi, which cause various clinical diseases. Considering the resistance of some Candida species to conventional antifungal agents, treatment of such cases may be challenging and complicated. The purpose of this study was to evaluate and compare the antifungal activities of Euphorbia macroclada latex and fluc...

متن کامل

Expression of Lanosterol 14-Demethylase (ERG11) Gene of Three-Drug Combinations in Candida albicans

Patients with impaired immunity are at particular risk of infections with Candida albicans. Antifungal drugs such as azoles commonly used for candidiasis treatment, but drug resistance is one of the most common problems for public health. The aim of this study was to evaluate the expression of lanosterol 14-demethylase (ERG11) gene for three-drug combinations in C. albicans. Disk diffusion and ...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره   شماره 

صفحات  -

تاریخ انتشار 2014